Route 2 is similar to other early-region routes, being forested and featuring some of the player's first Trainer battles. In this case, there are four, the fourth of which is against Bianca, one of the player's rivals. To the south an item can be found, after using Strength on a large boulder. From the Accumula Town entrance, there are two paths. The player will then have to travel through a patch of tall grass. Upon exiting the patch of grass, the player will meet another Trainer. The player can then choose to go south and jump over the ledge to travel back to Accumula Town. After defeating the Trainers situated along a small path that travels eastwards, the player will have to cross another patch of tall grass. After traveling north through the grass, the player can either choose to continue north to enter Striaton City, jump over ledges to go back to Accumula Town, walk east to go up stairs to obtain an item, or walk west to obtain an item after using Cut.
In Black 2 and White 2, this route is one of the last routes the player can access. In addition to the higher levels of the Pokémon on the route, the player can also spot graffiti on the back of the northmost sign. It reads "Nothing ventured, nothing gained. Medals await the adventurous!" Once it is read, the player can earn the Graffiti Gazer medal.
Route 2 leaves Accumula Town from the east, yet the in-game maps show it going straight north.
A graphical artifact happens in this route: there are two buildings in the north of Accumula Town that should be seen from a section of the road, but they don't get rendered due to a process of clipping activated when exiting the gate to route 2. By cheating, it is possible to exit Accumula Town through the trees and view the buildings not clipped.
The sign at the northern end of the route (near Striaton City) simply says "Route 2." However, in Black 2 and White 2, reading the sign from the back displays the message that there is some graffiti on the back of the sign. Doing this earns the player the Graffiti Gazer Medal. After receiving the medal, it is no longer possible to read the graffiti, and reading the sign from either side will only read "Route 2."