In the third room, there is a scientist who will revive the fossils the player obtained at Mt. Moon and Pewter Museum of Science. After giving him the fossil, the player must leave the lab and return to claim their fossil Pokémon. There is also a third in-game trade here: Seel for a Ponyta in the Japanese Red and Green, international Red and Blue, and FireRed and LeafGreen; a Krabby for a Growlithe in the Japanese Blue; and Muk for Kangaskhan in Yellow.
When Ash and his friends arrived on Cinnabar Island in Riddle Me This, they had planned to visit the Pokémon Lab, although they were dismayed to discover that it, like the rest of Cinnabar Island, had turned into yet another tourist trap. Later, Team Rocket tried to steal all of the Pokémon in it, but were stopped and sent blasting off by Ash and his friends.
In the anime, the Pokémon Lab had a division for Fighting Pokémon.
The Lab reappeared in the Yellow chapter, where it was revealed to be connected to the Cinnabar Gym, working as Blaine's personal laboratory. Among other things, it was shown to contain a special tank meant for Mewtwo.