Boss Trainers do not have unique sprites like most other Trainer classes, instead appearing as other classes with the Boss Trainer title. They have Pokémon at slightly higher levels and more powerful Pokémon than the other Trainers in the Area; the Bosses of later areas even use legendary Pokémon. One notable Boss Trainer is Benga, Alder's grandson, who is the leader of the tenth and final section of the facility during the initial visit.
Each Boss Trainer has Pokémon with 3 levels more and 600 more than previous Trainer in Area order.
With exception of Benga, each Boss Trainer's first letter in their name is based on the alphabetical order in each numbered Area order. In the Japanese version, their first letter is based on the katakana in vowel "a" column order instead.